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<DIV><FONT size=2 face=Arial>man irgi atrodo, kad greiciau jau smegenine i
sloika su spiritu ides ir laidus prijungs, nei kad sustabdytiems cheminiams
lasteliu procesams "pause/resume" padaryti sugebes.</FONT></DIV>
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<DIV>"Necris" <<A href="mailto:abc@def.aef">abc@def.aef</A>> wrote in
message <A
href="news:hjuh29$878$1@trimpas.omnitel.net">news:hjuh29$878$1@trimpas.omnitel.net</A>...</DIV>
<DIV>"The risk of inadequate preservation may be ruled out completely within
another decade if techniques for transplantable organ banking can be adapted
to achieve reversible preservation of the brain".</DIV>
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<DIV>"Necris" <<A href="mailto:abc@def.aef">abc@def.aef</A>> wrote in
message <A
href="news:hjugtg$7v1$1@trimpas.omnitel.net">news:hjugtg$7v1$1@trimpas.omnitel.net</A>...</DIV>
<DIV><FONT size=2 face=Arial>ale jie vystosi labai sparciai su savo tom
vitrification, per 20</FONT></DIV>
<DIV><FONT size=2 face=Arial>metu tipo labai dideli progresa
padare, yra ten kazkur mikroskopines</FONT></DIV>
<DIV><FONT size=2 face=Arial>fotkes atsildytu smegeneliu, tipo nesimato
jokio damage :)</FONT></DIV>
<DIV><FONT size=2 face=Arial>Speju po kokiu 40 metu dar kelis kart
patobules.</FONT></DIV>
<DIV><FONT size=2 face=Arial>Tik baisiausia, jei po poros simtu metu
prikeltu kazkokiu tai daunu :(</FONT></DIV>
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<DIV>"flegmatoid" <<A
href="mailto:flegmatoid@gmail.com">flegmatoid@gmail.com</A>> wrote in
message <A
href="news:hjudke$2mc$1@trimpas.omnitel.net">news:hjudke$2mc$1@trimpas.omnitel.net</A>...</DIV>
<DIV><FONT size=2 face=Arial>pasiskaitinejus "FAQ for Scientist" skyreli,
pernelyg optimistiskai nenuteikia:</FONT></DIV>
<DIV><FONT size=2 face=Arial></FONT><FONT size=2
face=Arial></FONT> </DIV>
<DIV><FONT size=2 face=Arial>"Although much interest has attended the
cryopreservation of immature neurons for subsequent therapeutic
intracerebral transplantation, there are no reports on the
cryopreservation of organized adult cerebral tissue slices of potential
interest for pharmaceutical drug development. We report here the first
experiments on cryopreservation of mature rat transverse hippocampal
slices. Freezing at 1.2 degrees C/min to -20 degrees C or below using 10
or 30% v/v glycerol or 20% v/v dimethyl sulfoxide yielded extremely poor
results. Hippocampal slices were also rapidly inactivated by simple
exposure to a temperature of 0 degree C in artificial cerebrospinal fluid
(aCSF). This effect was mitigated somewhat by 0.8 mM vitamin C, the use of
a more "intracellular" version of aCSF having reduced sodium and calcium
levels and higher potassium levels, and the presence of a 25% w/v mixture
of dimethyl sulfoxide, formamide, and ethylene glycol ("V(EG) solutes";
Cryobiology 48, pp. 22-35, 2004). It was not mitigated by glycerol,
aspirin, indomethacin, or mannitol addition to aCSF. When RPS-2
(Cryobiology 21, pp. 260-273, 1984) was used as a carrier solution for up
to 50% w/v V(EG) solutes, 0 degree C was more protective than 10 degrees
C. Raising V(EG) concentration to 53% w/v allowed slice vitrification
without injury from vitrification and rewarming per se, but was much more
damaging than exposure to 50% w/v V(EG). <STRONG>This problem was overcome
by using the analogous 61% w/v VM3 vitrification solution (Cryobiology 48,
pp. 157-178, 2004) containing polyvinylpyrrolidone and two extracellular
"ice blockers." With VM3, it was possible to attain a tissue K(+)/Na(+)
ratio after vitrification ranging from 91 to 108% of that obtained with
untreated control slices. Microscopic examination showed severe damage in
frozen-thawed slices, but generally good to excellent ultrastructural and
histological preservation after vitrification.</STRONG> Our results
provide the first demonstration that both the viability and the structure
of mature organized, complex neural networks can be well preserved by
vitrification. These results may assist neuropsychiatric drug evaluation
and development and the transplantation of integrated brain regions to
correct brain disease or injury."</FONT></DIV>
<DIV><FONT size=2 face=Arial></FONT><FONT size=2
face=Arial></FONT> </DIV>
<DIV><FONT size=2 face=Arial>reziume - uzsaldyt iseina su nedideliais
nuostoliais(tipo), bet atsilus nieko nelieka.</FONT></DIV>
<DIV><FONT size=2 face=Arial></FONT> </DIV>
<DIV><FONT size=2 face=Arial>"bzik" <</FONT><A
href="mailto:bzik@bzik.bzik"><FONT size=2
face=Arial>bzik@bzik.bzik</FONT></A><FONT size=2 face=Arial>> wrote in
message </FONT><A href="news:hjphf2$aqc$1@trimpas.omnitel.net"><FONT
size=2 face=Arial>news:hjphf2$aqc$1@trimpas.omnitel.net</FONT></A><FONT
size=2 face=Arial>...</FONT></DIV><FONT size=2 face=Arial>> sakyciau
brangiai parduodama iliuzija. Jei jie atgaivintu gyvenimui pries koki
desimtmeti uzsaldyta kuneli, gal butu itikinamiau. <BR>> asmeniskai nei
amzinam gyvenimui, nei atsigaivinimui po simto metu nematau prasmes :)
isivaizduok 19a 60-meti siandieninej aplinkoj. Gal prie kazko sugebetu
adaptuotis, bet visumoj liktu antrarusiu padaru aplinkiniu pajuokai. O gal
po pirmo pasimaitinimo desra su E kojas pakratytu :)<BR>> <BR>>
"Necris" <</FONT><A href="mailto:abc@def.aef"><FONT size=2
face=Arial>abc@def.aef</FONT></A><FONT size=2 face=Arial>> wrote in
message </FONT><A href="news:hjpg23$899$1@trimpas.omnitel.net"><FONT
size=2 face=Arial>news:hjpg23$899$1@trimpas.omnitel.net</FONT></A><FONT
size=2 face=Arial>...<BR>>> $80.000 uz galva, $150.000 uz visa kuna.
Ka manot?:)<BR>>> MKN, ideja pakankamai moksliskai pagrista ir
isvystyta,<BR>>> kad butu galima tiketis kazkokiu rezultatu (jei
nebankrutuos<BR>>> po poros simtu metu:)<BR>></FONT>
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