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<DIV><FONT size=2 face=Arial>pasiskaitinejus "FAQ for Scientist" skyreli,
pernelyg optimistiskai nenuteikia:</FONT></DIV>
<DIV><FONT size=2 face=Arial></FONT><FONT size=2 face=Arial></FONT> </DIV>
<DIV><FONT size=2 face=Arial>"Although much interest has attended the
cryopreservation of immature neurons for subsequent therapeutic intracerebral
transplantation, there are no reports on the cryopreservation of organized adult
cerebral tissue slices of potential interest for pharmaceutical drug
development. We report here the first experiments on cryopreservation of mature
rat transverse hippocampal slices. Freezing at 1.2 degrees C/min to -20 degrees
C or below using 10 or 30% v/v glycerol or 20% v/v dimethyl sulfoxide yielded
extremely poor results. Hippocampal slices were also rapidly inactivated by
simple exposure to a temperature of 0 degree C in artificial cerebrospinal fluid
(aCSF). This effect was mitigated somewhat by 0.8 mM vitamin C, the use of a
more "intracellular" version of aCSF having reduced sodium and calcium levels
and higher potassium levels, and the presence of a 25% w/v mixture of dimethyl
sulfoxide, formamide, and ethylene glycol ("V(EG) solutes"; Cryobiology 48, pp.
22-35, 2004). It was not mitigated by glycerol, aspirin, indomethacin, or
mannitol addition to aCSF. When RPS-2 (Cryobiology 21, pp. 260-273, 1984) was
used as a carrier solution for up to 50% w/v V(EG) solutes, 0 degree C was more
protective than 10 degrees C. Raising V(EG) concentration to 53% w/v allowed
slice vitrification without injury from vitrification and rewarming per se, but
was much more damaging than exposure to 50% w/v V(EG). <STRONG>This problem was
overcome by using the analogous 61% w/v VM3 vitrification solution (Cryobiology
48, pp. 157-178, 2004) containing polyvinylpyrrolidone and two extracellular
"ice blockers." With VM3, it was possible to attain a tissue K(+)/Na(+) ratio
after vitrification ranging from 91 to 108% of that obtained with untreated
control slices. Microscopic examination showed severe damage in frozen-thawed
slices, but generally good to excellent ultrastructural and histological
preservation after vitrification.</STRONG> Our results provide the first
demonstration that both the viability and the structure of mature organized,
complex neural networks can be well preserved by vitrification. These results
may assist neuropsychiatric drug evaluation and development and the
transplantation of integrated brain regions to correct brain disease or
injury."</FONT></DIV>
<DIV><FONT size=2 face=Arial></FONT><FONT size=2 face=Arial></FONT> </DIV>
<DIV><FONT size=2 face=Arial>reziume - uzsaldyt iseina su nedideliais
nuostoliais(tipo), bet atsilus nieko nelieka.</FONT></DIV>
<DIV><FONT size=2 face=Arial></FONT> </DIV>
<DIV><FONT size=2 face=Arial>"bzik" <</FONT><A
href="mailto:bzik@bzik.bzik"><FONT size=2
face=Arial>bzik@bzik.bzik</FONT></A><FONT size=2 face=Arial>> wrote in
message </FONT><A href="news:hjphf2$aqc$1@trimpas.omnitel.net"><FONT size=2
face=Arial>news:hjphf2$aqc$1@trimpas.omnitel.net</FONT></A><FONT size=2
face=Arial>...</FONT></DIV><FONT size=2 face=Arial>> sakyciau brangiai
parduodama iliuzija. Jei jie atgaivintu gyvenimui pries koki desimtmeti
uzsaldyta kuneli, gal butu itikinamiau. <BR>> asmeniskai nei amzinam
gyvenimui, nei atsigaivinimui po simto metu nematau prasmes :) isivaizduok 19a
60-meti siandieninej aplinkoj. Gal prie kazko sugebetu adaptuotis, bet visumoj
liktu antrarusiu padaru aplinkiniu pajuokai. O gal po pirmo pasimaitinimo desra
su E kojas pakratytu :)<BR>> <BR>> "Necris" <</FONT><A
href="mailto:abc@def.aef"><FONT size=2 face=Arial>abc@def.aef</FONT></A><FONT
size=2 face=Arial>> wrote in message </FONT><A
href="news:hjpg23$899$1@trimpas.omnitel.net"><FONT size=2
face=Arial>news:hjpg23$899$1@trimpas.omnitel.net</FONT></A><FONT size=2
face=Arial>...<BR>>> $80.000 uz galva, $150.000 uz visa kuna. Ka
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isvystyta,<BR>>> kad butu galima tiketis kazkokiu rezultatu (jei
nebankrutuos<BR>>> po poros simtu metu:)<BR>></FONT></BODY></HTML>